How are isolates and reference strains stored?

Pure isolates are typically stored long-term in tryptic soya broth with 8% DMSO or using microbeads both at -80ºC.

Frozen sputum is cut and stored frozen at -80 ºC, some spun supernatant also stored at -80 ºC is also available (most utility for investigation of inflammatory markers).

BAL spun supernatant is stored at -80 (most utility for investigation of inflammatory markers).